Answer questions using research article.

Learning Goal: I’m working on a biology question and need an explanation and answer to help me learn.

The overall goal of the article is to develop a phosphospecific flow cytometry-based assay to measure the effects of the compound library on signaling pathways. Identified hits were then confirmed in their pathway selectivity assays and finally lead compound selectivity was confirmed in vivo in mice. With this background, answer the following questions pertaining to each of the figures in the journal article (20 points):

  1. Fig 1: What cells were used in the primary screening, what simulants were used to induce phosphorylation of p38 and pSTAT1 and what was the outcome. How did they validate the assay – 2 point
  2. Fig 2: Describe briefly (step-wise description) how the authors performed the screening of the NCI natural product library (what stimulants were used and what signaling pathways were they targeting/measuring (2 points). To identify the hits what threshold did they use and how did multiparameter kinase pathway screening help identify pathway selective inhibitors (2 point)
  3. Fig 3: Describe how validation of the hits identified from initial screen was performed and what was the outcome (2 points)
  4. Fig 4: Screening was performed in heterogeneous primary cell populations with a goal to identify: i) Pathway specific inhibitors; ii) cell-type specific inhibitors and iii) patterns of pathway and cell type druggability. As related to the figure 4, answer the following questions
    1. What was the origin (organ/tissue) of the primary cells and what markers were used to identify the different cell population;
    2. How did the authors demonstrate quantitative nature of the phosphoflow platform;
    3. What is Fluorescence cell barcoding and what is the advantage of its use;
    4. What is selectivity factor (SI) and how did they apply it to interpret the data in Fig. 4c,d. (4 points).
  5. Fig. 5: Briefly describe the experiment and outcome of the results that lead to the identification of pathway selective compound (2 points)
  6. Fig. 6 & 8: What experiment and results lead the authors to believe that the inhibitor Streptonigrin is a cell-type selective compound. What was the outcome of evaluating this compound in vivo in mice (4 points)
  7. Fig. 7: What is selectivity factor clustering and how did application of this statistical tool help them identify pathways and cell types that are druggable (2 points)

Please if you cant read articles and understand whats going on do not pick question. Each question must be 4-6 value sentences long minimum.

Get 20% Discount on This Paper
Pages (550 words)
Approximate price: -

Try it now!

Get 20% Discount on This Paper

We'll send you the first draft for approval by at
Total price:
$0.00

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

Paper Helper has assembled a team of highly skilled writers with diverse experience in the online writing circles. Our aim is to become a one stop shop for all your Academic/ online writing. Check out below our amazing service!

Essays

Essay Writing Services

At Paper Helper, we prioritize on all aspects that creates a good grade such as impeccable grammar, proper structure, zero-plagiarism, and conformance to guidelines. The principal purpose of essay writing is to present the author's evaluation concerning a singular subject about which they have made. Since Professionalism is the mother of every success, try our team of experienced writers in helping you complete your essays and other assignments.

Admissions

Admission Papers

You have been trying to join that prestigious institution you long yearned for, but the hurdle of an admission essay has become a stumbling block. We have your back, with our proven team that has gained invaluable experience over time, your chance of joining that institution is now! Just let us work on that essay.How do you write an admission essay? How do you begin the essay? For answers, try Quality Custom Writers Now!

Editing

Editing and Proofreading

Regardless of whether you're pleased with your composing abilities, it's never an impractical notion to have a second eye go through your work. The best editing services leaves no mistake untouched. We recognize the stuff needed to polish up a writing; as a component of our editing and proofreading, we'll change and refine your write up to guarantee it's amazing, and blunder free. Our group of expert editors will examine your work, giving an impeccable touch of English while ensuring your punctuation and sentence structures are top-notch.

Coursework

Technical papers

We pride ourselves in having a team of clinical writers. The stringent and rigorous vetting process ensures that only the best persons for job. We hire qualified PhD and MA writers only. We equally offer our team of writers bonuses and incentives to motivate their working spirit in terms of delivering original, unique, and informative content. They are our resources drawn from diverse fields. Therefore your technical paper is in the right hands. Every paper is assessed and only the writers with the technical know-how in that field get to work on it.

Coursework

College Essay Writing

If all along you have been looking for a trustworthy college essay service provider that provides superb academic papers at reasonable prices, then be glad that you search has ended with us. We are your best choice! Get high-quality college essay writing from our magnificent team of knowledgeable and dedicated writers right now!

Coursework

Quality Assignment/Homework Help

We give the students premium quality assignments, without alarming them with plagiarism and referencing issues. We ensure that the assignments stick to the rules given by the tutors. We are specific about the deadlines you give us. We assure you that you will get your papers well in advance, knowing that you will review and return it if there are any changes, which should be incorporated.

togel macau togel terbesar bocoran admin jarwo data macau